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ricF [2018-05-30 11:35:33]
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ricF [2018-05-30 11:35:33]

subunit of the regulatory iron-sulfur containing [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex, antagonist of biofilm repression by [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR], control of the [SW|phosphorelay]
locus
BSU14990
pI
5.01
mw
16.77 kDa
protein length
149 aa Sequence Blast
gene length
447 bp Sequence Blast
function
regulation of biofilm formation and the phosphorelay
product
subunit of the regulatory iron-sulfur containing [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex
essential
no
synonyms
ylbF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.5|Effectors of RNA degradation]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.6|Other protein controlling the activity of the phosphorelay]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.5|Other proteins required for biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.6|Other protein controlling the activity of the phosphorelay]

    • Gene

    Coordinates
    1,568,420 → 1,568,869

    Phenotypes of a mutant

  • strongly reduced [SW|genetic competence], strongly reduced [SW|sporulation] [Pubmed|23490197]

    • The protein

    Catalyzed reaction/ biological activity

  • the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex stimulates [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A] phosphorylation in the [category|SW 4.2.2|phosphorelay] [pubmed|27501195]

    • [SW|Localization]

  • cytoplasm [pubmed|27501195]

    • Expression and Regulation

    Operons

    genes
    [gene|6C9A092F38739A3759793EF8B496569CD02C2E3F|ylbF]
    description
    [Pubmed|22383849]
    view in new tab

    genes
    [gene|6C9A092F38739A3759793EF8B496569CD02C2E3F|ylbF]-[gene|FBD8F0EB241A35747E6398C9AA30D71D6D17CE99|ylbG]-[gene|65FA4B3738C2A5995F019099743C782ADD15C338|ylbH]-[gene|6DAAEF20B68139A732FDCDABD0C420A0C928D373|ylbI]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • BKE14990 (Δ[gene|6C9A092F38739A3759793EF8B496569CD02C2E3F|ylbF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14990 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG
  • BKK14990 (Δ[gene|6C9A092F38739A3759793EF8B496569CD02C2E3F|ylbF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG

    • References

  • 10712692,15661000,23490197,15175311,26434553,28295778,27501195